ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. Table of Contents. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. for ValitaTiter Plus standards read on Molecular Devices readers. Ltd. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Maximum signal with lowest background. ATP standard curves read on SpectraMax microplate readers. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. 7°C (gradient heating design). 1. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Fluorescence Microplate Readers. Figure 1. We use Spectramax M5 microplate reader in our lab. 0 out of. Western blot analyses. 6 for further experiment, so I'm confused which i would choose for OD value determination. SpectraMax® Aquamax. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. It cannot be used for luminescence measurements. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. Temperature control range from Ambient +4º to 50ºC. Molecular Devices. • Tel. keeping with best practices, reader performance should. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 00. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. SpectraMax® M5 Multi-Mode Microplate Reader (cat. We would like to show you a description here but the site won’t allow us. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. The acceptance. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. The following document is intended to demonstrate setup of this instrument. 2. 314 low lamp level warning. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Incubation. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax 250 User. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. 00 USD. Microplate Readers Biotek Synergy H1 Hybrid Microplate. 1. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Cell viability was quantified by measuring the absorbance of the solution at 570 nm using a SpectraMax® M5 microplate reader (Molecular Devices). The cells were harvested on ice and. Depending on which. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. The system. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] or greater. 0 μL) instead of the sample solution was applied as control group, and. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. Microplate Readers include the M3, M4, M5, and M5e models. When a feature or capability applies to only certain readers, this exception is noted. The following document is intended to demonstrate setup of this instrument. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. SpectraMax Lmicroplate luminometer. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. . Maximum signal with lowest background. Optical absorbance. Western blotting assays were conducted according to previous studies . on the SpectraMax Multi-Mode Microplate Readers by issuing a single. 00 USD. 2. SpectraMax iD5 Multi-Mode Microplate Reader. Ltd. The plate reader can measure multiple readouts, including UV and visible absorbance. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). D. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. The assay window spanned four orders of magnitude. #SpectraMax L Config) Methods TIGIT/CD155 Blockade Bioassay. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Figure 1. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 2. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. metrically at 540 nm using a microplate reader (SpectraMax 384 Plus, Molecular Devices Corp. Add to Cart. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. Thank you!. As. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. The raw data from each individual experiment were normalized to vehicle-treated HK-2. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. B: Selection of Excitation Wavelength. • Tel. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 3 1. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Cuvette port functionality can also be controlled using SoftMax Pro Software. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax Spectrofluorometers. Introduction. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Molecular Devices Spectramax M5 Microplate Reader. FlexStation™ III. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. spectramax m5 multi. ® reader to the computer. 312 gain calibration failed. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). Add to Cart. used. e. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideFluorescence Microplate Reader Comparison Chart. This application note describes how to. 1. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. Offer Ends In: 44 Days 10: 22: 56. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Please refer to the Obsolete Microplate Reader Models for more information. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 2 only). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SDS-PAGE Assay. B: Selection of Excitation Wavelength. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Table of Contents. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The CellTiter-Glo assay from Promega uses luciferase. HTRF-compatible SpectraMax Multi-Mode Readers. Microplate reader settings. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax M2 and M2e are versatile and reliable. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The calculated LOQ values were 0. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Linear, Orbital Slow, and Orbital Fast. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Supplier: Molecular Devices. 81). However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. both cuvette-based and microplate reader applications on the same instrument. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Ferguson, Ph. M3-M5e_manual. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. When a feature or capability applies to only certain readers, this exception is noted. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Equal amount of proteins were loaded and resolved using denaturing gel and analyzed by western blot using antibodies recognizing Akt, rS6, mOTR and. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. microplate reader. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. edu) to be added as a trained user in iLab. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Download PDF. FlexStation™ II. Also refer to this Multimode Reader Comparison Chart . Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Page 28 Chapter 5: Read Modes and Read Types 1. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 3 Version 7. For readers without a cuvette port, these options are not shown in the software. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. both cuvette-based and microplate reader applications The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). In brief, RAW 264. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. SpectraMax M5 Multi-Mode Microplate Reader. , USA). Materials. FlexStation™ I. SpectraMax® ABS. The PBS solution (10. The day before assay, assay buffer was prepared as described in the product technical manual. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 0 . The cells were then treated to induce apoptosis. View the Molecular Devices website. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Has anyone had problems with the built-in computer of the Spectramax ID3. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. 2. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Catalog Number: (89212-396) Supplier: Molecular Devices. Recommended Optics Wavelength (nm) Wavelength. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. A: Microplate Preparation. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Western blotting assay. Temperature variation ± 0. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. No filter change with dual monochromator optics. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. 0 μL) instead of the enzyme solution was used as the sample blank group. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 7°C (gradient heating design). SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. Figure 1. M3 M4 M5 M5e LM1 v<nnn>. 6% for the on-board optics (monochromators). This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Standard features include a. When a. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. M3-M5e_manual. When a The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Spectramax M5 microplate reader: Molecular Devices: N/A:. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). Molecular Devices SpectraMax® series microplate readers. Wide range of applications. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. Example: [Drawer]. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. We would like to show you a description here but the site won’t allow us. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. Access this by going to Help >. SpectraMax M5e Multi-Mode Microplate Reader. analysis, controls the SpectraMax Multi-Mode Microplate Readers. Maximum signal with lowest background. Parallel negative control assays had primary, secondary, or both antibodies omitted. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax® M5. FlexStation™ III. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax® Aquamax. Our absorbance plate readers feature our PathCheck Sensor. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. The concentration of antibodies was determined by using a spectrophotometer from Puxi General Instrument Co. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 1** Version 7. Do not touch the clear surfaces of the cuvette. Small, unbound molecules rotate rapidly in. 2. free tracer decreases, resulting in. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The chamber contains springs that position the cuvette in the proper alignment for a read. Add to Cart. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 303 unable to cal dark current. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. A: Microplate Preparation. book Page 7 Wednesday, July 7, 2010 2:09 PM. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The readings with excitation at 540 nm and emission at 600 nm represented the amount of phosphorylated IκB-α in the cells. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. For a higher . Italic and boldface type are used for emphasis. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. book Page 7 Wednesday, July 7, 2010 2:09 PM. e. Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. p. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Fill a clean plate with water. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 1 2. 1–0. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. 7 cells were co-transfected with 0. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Page 28 Chapter 5: Read Modes and Read Types 1. 04 to 3. AquaMax® 4000. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. RAW 264. The assay applies fluorescence polarization to quantify Fc-containing IgG. Five preferred modes for diverse bioresearch applications. However, when a specific probe is not available, or it causes off-target effects on the. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 1. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. (The i3x and Paradigm readers require the. 5. Our absorbance plate readers feature our PathCheck Sensor technology and. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The absorbance value of each reaction solution was measured at 405 nm by a Molecular Device SpectraMax M5 microplate reader (Sunnyvale, CA, USA). IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. STAKMAX. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). FlexStation™ I. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. Click SoftMax Pro Software User Guide. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. SpectraMax Microplate Readers. Shop now. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. View the Molecular Devices website. l. 5°C at 37°C. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. ,. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader.